Composite

Part:BBa_K4688006:Design

Designed by: Xiaoxin Xuan   Group: iGEM23_FZPt-CHINA   (2023-10-06)


FUCO2-GFP-expression


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1706
    Illegal SpeI site found at 1936
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1706
    Illegal SpeI site found at 1936
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1706
    Illegal BglII site found at 1
    Illegal BamHI site found at 3438
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1706
    Illegal SpeI site found at 1936
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1706
    Illegal SpeI site found at 1936
    Illegal NgoMIV site found at 1788
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization and gene expression efficiency.


Source

FUCO2 comes from chromosome 19 of the Phaeodactylum tricornutum genome.GFP comes from aequoreavictoria.

References